DIAGNOSTIC POSSIBILITIES OF ANALYSIS OF THE MAP OF LINEAR BIREFRINGENCE OF THE CRYSTAL FRACTION OF VITREOUS BODY FOR ACCURATE DETERMINATION OF THE TIME SINCE DEATH

Authors

  • Yuliia Sarkisova Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine
  • Viktor Bachynskіy Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine
  • Alina Palamar Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine
  • Nadya Palibroda Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine and Infectious Diseases Department, Ukraine
  • Maryna Patratii Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

DOI:

https://doi.org/10.12955/pmp.v1.103

Keywords:

Post-mortem interval, vitreous body, polarization.

Abstract

Introduction: This paper discusses the possibility of polarization microscopic tomography of polycrystalline structure of vitreous body (VB) for use in forensics and in determining the time since death (TSD).

Objectives: The purpose of this study was to develop a new set of forensic criteria to enhance the functionality of the high-precision definition of TSD over a long period of time according to polarization microscopic tomography of the polycrystalline structure of the VB of the human eye by statistical and wavelet analysis.

Results: We obtained the numerical values of the change in the magnitude of the statistical moments of the 1-4 orders, which characterize the coordinate distributions of the magnitude of the linear birefringence (LB) of the polycrystalline component of the VB by the magnitude of the TSD. The results from our research illustrate the differences between the optical anisotropy of fibrillar collagen networks of VB layers with different TSD. The sensitivity range (36 hours) and accuracy (15 minutes) of the method of polarization tomography of the LB distributions of the polycrystalline component of the VB layers in the determined TSD were established.

Conclusion: These results confirm the experimental processes outlined accurately determine the time of death. In turn, will provide scientific evidence, specifications thereof, and objective expert opinion.

Author Biographies

Yuliia Sarkisova, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Viktor Bachynskіy, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Alina Palamar, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Nadya Palibroda, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine and Infectious Diseases Department, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine and Infectious Diseases Department, Chernivtsi

Maryna Patratii, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine and Infectious Diseases Department, Chernivtsi, Ukraine

References

Bonete, G. P., Legaz, I., Martínez, C. P., Cárceles, M. D. P., & Luna, A. (2017). Biochemical correction factors to estimation of post-mortem interval in vitreous humor. Romanian Journal of Legal Medicine, 25(2), 201-204. doi: 10.4323/rjlm.2017.201

Duan, W. C., Lan, L. M., Guo, Y. D., Zha, L., Yan, J., Ding, Y. J., & Cai, J. F. (2018). Research Progress of Vitreous Humor Detection Technique on Estimation of Postmortem Interval. Fa Yi Xue Za Zhi, 34(1), 49-54. doi: 10.3969/j.issn.1004-5619.2018.01.010

Garcia, P. T., Gabriel, E. F., Pessôa, G. S., Júnior, J. C. S., Mollo Filho, P. C., Guidugli, R. B., ... & Coltro, W. K. (2017). Paper-based microfluidic devices on the crime scene: A simple tool for rapid estimation of post-mortem interval using vitreous humour. Analytica Chimica Acta, 974, 69-74. doi: 10.1016/j.aca.2017.04.040

Garazdyuk, M. S., Bachinskyi, V. T., Vanchulyak, O. Ya., Ushenko, A. G., Dubolazov, O. V., Gorsky, M. P. (2016). Polarization-phase images of liquor polycrystalline films in determining time of death. Applied Optics, 55(12), B67-B71. doi: 10.1364/AO.55.000B67

Go, A., Shim, G., Park, J., Hwang, J., Nam, M., Jeong, H., & Chung, H. (2019). Analysis of hypoxanthine and lactic acid levels in vitreous humor for the estimation of post-mortem interval (PMI) using LC–MS/MS. Forensic Science International, 299, 135-141. doi: 10.1016/j.forsciint.2019.03.024

Scrivano, S., Sanavio, M., Tozzo, P., & Caenazzo, L. (2019). Analysis of RNA in the estimation of post-mortem interval: a review of current evidence. International Journal of Legal Medicine, 133, 1629-1640.

Ushenko, A. G., Dubolazov, A. V., Ushenko, V. A., Ushenko, Yu. A., Sakhnovskiy, M. Yu., Sidor, M., … Lakusta, I. I. (2016). Methods and means of Stokes-polarimetry microscopy of optically anisotropic biological layers. Proceedings of SPIE, 10010, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies, VIII, 100100M. doi: https://doi.org/10.1117/12.2242628

Ushenko, V. A., Prysyazhnyuk, V. P., Dubolazov, O. V., Karachevtsev, A. O., Olar, O. I., Olar, O. V., … Savich, V. O. (2015). Polarization-correlation microscopy of human liquid polycrystalline films in infertility diagnosis. Proceedings of SPIE, 9599, Applications of Digital Image Processing, XXXVIII, 959922. doi: https://doi.org/10.1117/12.2187448

Vanchulyak, O., Ushenko, Y., Galochkin, O., Sakhnovskiy, M., Kovalchuk, M., Dovgun, A. … Bodnar, G. (2019). Azimuthal fractalography of networks of biological crystals. Proceedings of SPIE, 11105, Novel Optical Systems, Methods, and Applications, XXII, 1110517. doi: https://doi.org/10.1117/12.2529337

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Published

2020-11-16

How to Cite

Sarkisova, Y. ., Bachynskіy V. ., Palamar, A. ., Palibroda, N. ., & Patratii, M. . (2020). DIAGNOSTIC POSSIBILITIES OF ANALYSIS OF THE MAP OF LINEAR BIREFRINGENCE OF THE CRYSTAL FRACTION OF VITREOUS BODY FOR ACCURATE DETERMINATION OF THE TIME SINCE DEATH. Proceedings of CBU in Medicine and Pharmacy, 1, 82-89. https://doi.org/10.12955/pmp.v1.103