TO DEVELOP FORENSIC CRITERIA FOR THE DIFFERENTIAL DIAGNOSIS OF INTRACEREBRAL HEMORRHAGE OF TRAUMATIC AND NON-TRAUMATIC ORIGIN USING MUELLER-MATRIX MICROSCOPY OF LINEAR DICHROISM

Authors

DOI:

https://doi.org/10.12955/pmp.v2.165

Keywords:

traumatic intracranial hemorrhage, non-traumatic intracranial hemorrhage, cause of death, azimuthal-invariant, Mueller-matrix microscopy

Abstract

Differential diagnosis of intracerebral hemorrhage due to traumatic and non-traumatic origin is a challenging issue, especially in the absence of visible body injuries and other signs of violence. For a forensic expert-practitioner, the main thing is objectivity, accuracy, and speed of obtaining the result, which could fully satisfy the methods of laser polarimetry in the case of differential diagnosis of hemorrhages of traumatic and non-traumatic origin in the human brain matter.

The purpose: To develop forensic criteria for the differential diagnosis of intracerebral hemorrhage of traumatic and non-traumatic origin using Mueller-matrix microscopy of linear dichroism.

Materials and methods: The object of the study was brain matter samples, collected in 115 corpses of both sexes aged 22 to 86 years with accurately known causes of death. The causes of death included coronary artery disease, traumatic brain hemorrhage, ischemic brain infarction, and non-traumatic brain hemorrhages. Brain matter samples have been frozen rapidly, and histological sections have been made using a freezing microtome. The slices have been evaluated using the method of azimuthal-invariant Mueller-matrix microscopy, followed by mathematical and statistical processing of the results. 

Results: significant topographic heterogeneity of Mueller-matrix invariants of brain matter samples linear dichroism maps among all research groups have been found. Individual and significant variations of dispersion, asymmetry, and kurtosis magnitude have confirmed the heterogeneity of Mueller-matrix invariants. The difference of images and histograms among groups is explained by the presence of necrotic changes in the case of ischemic brain necrosis and blood cells in the brain matter samples. For the set of central statistical moments of the 1st, 3rd, and 4th orders, which characterize the distributions of circular dichroism, the method of MM-microscopy in the differentiation of samples of non-hemorrhage and hemorrhage groups reaches a satisfactory level - 78% - 84%.

Conclusions: Brain matter samples Muller-matrix microscopy of linear dichroism is effective for solving diagnostic problems of forensic medicine related to evaluating the cause of death from intracerebral hemorrhage of various origins. Diagnostic efficiency of Mueller-matrix mapping of polarization manifestations of linear dichroism method for intergroup differentiation of samples of deaths from traumatic hemorrhage (Group 2) and ischemic cerebral infarction (Group 3) reaches a satisfactory level of 79 - 84%.

References

Bachinsky, V., Vanchulyak, Y., Ushenko, A. G., Ushenko, Y. A., Dubolazov, A. V., Bykov, A., Hogan, B., & Meglinski, I. (2021). Diagnosis of Acute Coronary Insufficiency by the Method of Mueller Matrix Analysis of Myosin Myocardium Networks SpringerBriefs in Applied Sciences and Technology (pp. 53-87): Springer.

Bailey, E. L., Smith, C., Sudlow, C. L., & Wardlaw, J. M. (2012). Pathology of lacunar ischemic stroke in humans—a systematic review. Brain Pathology, 22(5), 583-591. doi:10.1111/j.1750-3639.2012.00575.x

Bertozzi, G., Maglietta, F., Sessa, F., Scoto, E., Cipolloni, L., Di Mizio, G., Salerno, M., & Pomara, C. (2020). Traumatic Brain Injury: A Forensic Approach: A Literature Review. Curr Neuropharmacol, 18(6), 538-550. doi:10.2174/1570159x17666191101123145

Finnie, J. W. (2016). Forensic Pathology of Traumatic Brain Injury. Vet Pathol, 53(5), 962-978. doi:10.1177/0300985815612155

Garazdiuk, M., Bachynskіy, V., Nechytailo, O., Garazdiuk, O., & Malanchuk, S. (2020). Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-traumatic Genesis. Proceedings of CBU in Medicine and Pharmacy, 1, 18-23. doi:10.12955/pmp.v1.92

Hao, S., & Wang, B. (2017). Editorial: Review on Intracerebral Haemorrhage: Multidisciplinary Approaches to the Injury Mechanism Analysis and Therapeutic Strategies. Curr Pharm Des, 23(15), 2159-2160. doi:10.2174/1381612823999170307150448

Kaur, P., & Sharma, S. (2018). Recent Advances in Pathophysiology of Traumatic Brain Injury. Curr Neuropharmacol, 16(8), 1224-1238. doi:10.2174/1570159x15666170613083606

Sivokorovskaya, N., Bachinsky, V., Vanchulyak, O., Ushenko, A., Dubolazov, A., Ushenko, Y., & Tomka, Y. (2020). Polarization-phase diagnostics of volume of blood loss. Paper presented at the Applications of Digital Image Processing XLIII.

Trifonyuk, L., Sdobnov, A., Baranowski, W., Ushenko, V., Olar, O., Dubolazov, A., Pidkamin, L., Sidor, M., Vanchuliak, O., Motrich, A., Gorsky, M., & Meglinski, I. (2020). Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues. Lasers Med Sci, 35(4), 877-891. doi:10.1007/s10103-019-02878-2

Ushenko, Y. A. (2011). Investigation of formation and interrelations of polarization singular structure and Mueller-matrix images of biological tissues and diagnostics of their cancer changes. J Biomed Opt, 16(6), 066006. doi:10.1117/1.3585689

Downloads

Published

2021-10-24

How to Cite

Garazdiuk, M. ., Bachynskyі V. ., Nechytailo, O. ., & Garazdiuk, O. . (2021). TO DEVELOP FORENSIC CRITERIA FOR THE DIFFERENTIAL DIAGNOSIS OF INTRACEREBRAL HEMORRHAGE OF TRAUMATIC AND NON-TRAUMATIC ORIGIN USING MUELLER-MATRIX MICROSCOPY OF LINEAR DICHROISM . Proceedings of CBU in Medicine and Pharmacy, 2, 21-24. https://doi.org/10.12955/pmp.v2.165
صندلی اداری سرور مجازی ایران Decentralized Exchange
فروشگاه اینترنتی صندلی اداری جوراب افزایش قد ژل افزایش قد