• Yanka Karamalakova Trakia University, Medical Faculty, Chemistry and Biochemistry, Stara Zagora
  • Iliana Koleva Trakia University, Medical Faculty, Obstetrics and Gynecology Clinic, Stara Zagora
  • Tzvetelin Georgiev Trakia University, Medical Faculty, Physiology, Pathophysiology and Pharmacology, Stara Zagora
  • Muhammad Akram Trakia University, Medical Faculty, Chemistry and Biochemistry, Stara Zagora
  • Galina Nikolova Trakia University, Medical Faculty, Chemistry and Biochemistry, Stara Zagora



LME, BLM, ROS, lung inflammations


This research investigates the probable effects of induced chronic (28 days) lung inflammations by Bleomycin (BLM) and its oxidative-toxicity protection by the aquatic extract of Lemna minor L. (LME). Balb/c male mice were  in every two days exposed to: (1) a controlled normal diet, (2) an LME treatment (120 mg/kg bwt, i.p.), (3) a BLM treatment (0.34 U/kg bwt, i.p.), and (4) an LME (120 mg/kg bwt, i.p.) administered two hours prior to the BLM. At the 30 experimental days of chronic BLM administration, the mice were sacrificed and fresh lung tissue was collected for biochemical determination and EPR analysis. The BLM treatment significantly increased the biochemical indices two-fold (SOD, CAT, MDA, TC) than controls. Furthermore, lung/alveolar cell experiments were performed to investigate the LME modulative and oxidative-protection effect. The results revealed that LME alone and in combination (LME + BLM) inhibited BLM expression by significantly reducing EPR-ascorbate (p < 0.05), ROS production (p < 0.05), and by enhancing enzymatic antioxidants. As a conclusion, our results indicated that chronic BLM toxicity and lung inflammation could be neutralized by long-term LME treatment. Therefore, LME + BLM prevented the detrimental impacts of BLM and have proved to have a potential therapeutic effect on the oxidative stress biomarkers, antioxidant enzymes and alleviation of lung inflammations.


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How to Cite

Karamalakova, Y. ., Koleva, I. ., Georgiev, T. ., Akram, M. ., & Nikolova, G. . (2021). PROTECTIVE ACTIVITY OF LEMNA MINOR L. IN CHRONIC BLEOMYCIN–INDUCED LUNG INFLAMMATION. Proceedings of CBU in Medicine and Pharmacy, 2, 60-65.