PHYTOCHEMICAL STUDY OF ENDEMIC SPECIES HELLEBORUS CAUCASICUS, HELLEBORUS ABCHASICUS AND FICARIA POPOVII SPREAD IN SOUTHERN COLCHIS

Authors

  • Medea Beridze Batumi Shota Rustaveli State University, Georgia
  • Aleko Kalandia Batumi Shota Rustaveli State University, Georgia
  • Indira Japaridze Batumi Shota Rustaveli State University, Georgia
  • Maia Vanidze Batumi Shota Rustaveli State University, Georgia
  • Natela Varshanidze Batumi Shota Rustaveli State University, Georgia
  • Nazi Turmanidze Batumi Shota Rustaveli State University, Georgia
  • Ketevan Dolidze Batumi Shota Rustaveli State University, Georgia
  • Inga Diasamidze Batumi Shota Rustaveli State University, Georgia
  • Eteri Jakeli Batumi Shota Rustaveli State University, Georgia

DOI:

https://doi.org/10.12955/pmp.v1.89

Keywords:

Phytochemistry, UPLC-MS, Ficaria Popovii, Helleborus caucasicus, Helleborus abchasicus

Abstract

There are 176 endemic plants spread in southern Colchis, of which 45  can be used for some medical treatments. The bioecology and detailed phytochemical content of some medicinal plant populations have not  been studied so far.

The research objectiveis to study the phytochemical content of endemic species of Helleborus caucasicus, Helleborus abchasicus and Ficaria popovii spread in southern Colchis.

The research method for the phytochemical content included separation analysis, whichwas performed byusing UPLC-MS (Waters Acquity QDa detector).

Three Steroidal glycosideswere isolated from the MeOH extract of the plants of Helleborus caucasicus and Helleborus abchasicus: Hellebrigenin-D-glucose, 20 – Hydroxyecdysone and Hydroxyecdysone – 3 glucoside. Two saponins (Hederagenin 3-O -α-L-arabino pyranoside, Hederagenin28-O-[α-L-rhamno-pyranosyl(1→4)-β-D-glucopyranosyl-(1→6)]β-D-lucopyranoside) and four flavonoids (kaempferol 3-O-β-ᴅ- (6ʺ-α-ʟ-rhamnopyranosyl)-glucopyranoside (nicotiflorin), apigenin 8-C-β-ᴅ-glucopyranoside (vitexin), luteolin 8-C-β-ᴅ-glucopyranoside (orientin), quercetin 3-O-rutinoside) were  isolated from the tubers and flowers of Ficaria Popovii.

Three Steroidal glycosides and Hydroxyecdysone -3 glucoside were isolated from the MeOH extract of Helleborus caucasicus.In addition, two saponins and four flavonoids were isolated from the tubers and leaves of FicariaPopovii.

Author Biographies

Medea Beridze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia

Aleko Kalandia, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of chemistry, Batumi, Georgia

Indira Japaridze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of chemistry, Batumi, Georgia

Maia Vanidze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of chemistry, Batumi, Georgia

Natela Varshanidze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia

Nazi Turmanidze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia

Ketevan Dolidze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia

Inga Diasamidze, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia

Eteri Jakeli, Batumi Shota Rustaveli State University, Georgia

Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Pharmacy, Batumi, Georgia

References

Dmitrieva, A. (1990). [in Russian], Key to flora of Adjara. Vol. II. Tbilisi: Metsniereba.

IUCN. (2006). Guidelines for using the IUCN Red List categories and criteria. Version 6.2. Retrieved from www.redlist.org/info/categories criteria.html.

Jakeli, E., Varshanidze, N., Diasamidze I., Dolidze K., Zarnadze N. (2018). Biodiversity of medical plants of wild flora in Ajara-South Colchis and their usage in folk medicine. 3-rd International Science Symposium “New Horizons in Science”, Proceeding Book., At Pristina, Cosovo. 80-96.

Ladislav, N., Ghuloom, H., Al-Hasawi N. (2019). Structural Features and Biological Activities of Bufadienolides. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kuwait University.

Manvelidze, Z., Memiadze, N., Kharazishvili, D., Varshanidze N. (2010). Diversity of floral area of Adjara. (List of wild grown plants species). Annalis of Agrarian science, 6 (2), 91-93.

Memiadze, N. (2004). Botanical and geographical survey of the endemics of Ajara-Lazeti flora. Bull. Georg. Acad. Sci. 169 (2), 341-343.

METLIN (2018). A Technology Platform for Identifying Knowns and Unknowns. Retrieved from https://metlin.scripps.edu.

Tomczyk, M., Gude, J., Sochacki, M. (2002). Zeitschrift fur Naturforschung Flavonoids from Ficaria verna Huds. C, Journal of Biosciences, 57(5-6):440-444 DOI: 10.1515/znc-2002-5-606 PMID: 12132681.

Varshanidze, N., Turmanidze, N., Dolidze, K., Zarnadze, N., Diasamidze, I., Epitashvili, T., Katcharava, T. (2018). Biodiversity of Medicinal Plants Containing Essential Oil and Their Spreading in Adjara. Universal Journal of Agricultural Research 6(3), 99-104 https://doi.org/10.13189/ujar.2018.060301.

Downloads

Published

2020-11-16

How to Cite

Beridze, M. ., Kalandia, A. ., Japaridze, I. ., Vanidze, M. ., Varshanidze, N. ., Turmanidze, N. ., Dolidze, K., Diasamidze, I. ., & Jakeli, E. . (2020). PHYTOCHEMICAL STUDY OF ENDEMIC SPECIES HELLEBORUS CAUCASICUS, HELLEBORUS ABCHASICUS AND FICARIA POPOVII SPREAD IN SOUTHERN COLCHIS. Proceedings of CBU in Medicine and Pharmacy, 1, 1-7. https://doi.org/10.12955/pmp.v1.89