ESTIMATION OF THE TIME SINCE DEATH BY USING THE POLARIZATION MULLER-MATRIX MICROSCOPY OF BIOLOGICAL TISSUE IN CASES OF ALCOHOL AND CARBON MONOXIDE POISONING

Authors

  • Ihor Ivaskevich Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine
  • Oleh Vanchuliak Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine
  • Viktor Bachynskіy Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine
  • Sviatoslava Yurniuk Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

DOI:

https://doi.org/10.12955/pmp.v1.96

Keywords:

Post-mortem interval, carbon monoxide poisoning, alcohol poisoning, polarization

Abstract

Introduction: This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning.

Objectives: The purpose of the study was to evaluate the diagnostic capabilities of the azimuthal-invariant Muller-matrix polarimetry technique of the polycrystalline constituent of BT and human body fluids in determining the TSD in cases of alcohol and carbon monoxide poisoning.

Results: A comprehensive experimental study of the diagnostic efficiency of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the brain, myocardium, adrenal glands, liver, and polycrystalline blood films from dead persons for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.A sensitivity range of 24 hours was determined using the method of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the myocardium for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.

Conclusion: The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

Author Biographies

Ihor Ivaskevich, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Oleh Vanchuliak, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Viktor Bachynskіy, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Forensic Medicine and Medical Law Department, Chernivtsi, Ukraine

Sviatoslava Yurniuk, Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Ukraine

Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Pharmacy Department, Chernivtsi, Ukraine

References

Gonnade, U., & Chavan, K. D. (2018). Study of early postmortem changes in skin for estimation of postmortem interval at pims, loni. Journal of Indian Academy of Forensic Medicine, 40(4), 396-400, doi: https://doi.org/10.5958/0974-0848.2018.00088.X

Hayden, J. (2019). Ethanol determination using automated analyzers: limitations and pitfalls. In A. Dasgupta, & J. Sepulveda, (eds.), Accurate Results in the Clinical Laboratory: A Guide to Error Detection and Correction (pp. 271-291). Elsevier.

Kim, H. H., Choi, S. C., Chae, M. K., & Min, Y. G. (2018). Neuroprotective effect of ethanol in acute carbon monoxide intoxication: A retrospective study. Medicine (Baltimore),97(1), doi: https://doi.org/10.1097/MD.0000000000009569

Rose, J. J., Wang, L., Xu, Q., McTiernan, C. F., Shiva, S., Tejero, J., & Gladwin, M. T. (2017). Carbon monoxide poisoning: pathogenesis, management, and future directions of therapy. American Journal of Respiratory and Critical Care Medicine, 195(5), 596-606, doi: https://doi.org/10.1164/rccm.201606-1275CI

Shaaban El-Raouf, A. A., Farrag, I. M., & Bayoumy, E. S. (2017). Estimation of early postmortem interval by biochemical changes in brain and liver of rats using some oxidant and antioxidant parameters. The Egyptian Journal of Forensic Sciences and Applied Toxicology, 17(1), 147-162, doi: https://doi.org/10.21608/EJFSAT.2017.46108

Tomé, M. C. F. (2017). Estimation of Postmortem Interval Based on Cell Death Progression in Biological Fluids (Masters dissertationthe Faculty of Medicine, University of Porto,Porto, Portugal.

Ushenko, A.G., Dubolazov, A.V., Ushenko, V.A., Ushenko, Yu.A., Pidkamin, L.Y., Soltys, I.V. … Pavlyukovich, N. (2016). Mueller-matrix mapping of optically anisotropic fluorophores of molecular biological tissues in the diagnosis of death causes. Proceedings of SPIE, 9971, Applications of Digital Image Processing, XXXIX, 99712L, doi: https://doi.org/10.1117/12.2237662

Ushenko, V. A., Prysyazhnyuk, V. P., Dubolazov, O. V., Karachevtsev, A. O., Olar, O. I., Olar, O. V., … Savich, V. O. (2015). Polarization-correlation microscopy of human liquid polycrystalline films in infertility diagnosis. Proceedings of SPIE, 9599, Applications of Digital Image Processing, XXXVIII, 959922,doi: https://doi.org/10.1117/12.2187448

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Published

2020-11-16

How to Cite

Ivaskevich, I. ., Vanchuliak, O. ., Bachynskіy V. ., & Yurniuk, S. . (2020). ESTIMATION OF THE TIME SINCE DEATH BY USING THE POLARIZATION MULLER-MATRIX MICROSCOPY OF BIOLOGICAL TISSUE IN CASES OF ALCOHOL AND CARBON MONOXIDE POISONING. Proceedings of CBU in Medicine and Pharmacy, 1, 41-47. https://doi.org/10.12955/pmp.v1.96